DNA

Part:BBa_K3860007:Design

Designed by: Marius Schnutenhaus   Group: iGEM21_RUBochum   (2021-09-30)


Monotreme Lactation Protein (MLP) from Ornithorhynchus anatinus


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 828
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 244
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 828
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 828
    Illegal AgeI site found at 589
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1202


Design Notes

The MLP from Ornithorhynchus anatinus was optimized for P. pastoris and a 6X His-tag was added.

Source

Genomic sequence Ornithorhynchus anatinus

References

https://www.ncbi.nlm.nih.gov/protein/AIG59189.1